Phosphorylated protein chip combined with artificial intelligence tools for precise drug screening
Katsuhisa Horimoto1
Yuki Suyama2
Tadamasa Sasaki2
Kazuhiko Fukui3
Lili Feng4
Meiling Sun5
Yamin Tang4
Yixuan Zhang6
Dongyin Chen7
Feng Han7
1.SOCIUM Inc.,Tokyo 1350064,Japan;International Medical Center,Saitama Medical University,Saitama 350-1298,Japan;Artificial Intelligence Research Center,National Institute of Advanced Industrial Science and Technology,Tokyo 1350064,Japan2.SOCIUM Inc.,Tokyo 1350064,Japan3.Department of Informatics and Data Science,Sanyo-Onoda City University,Yamaguchi 7560884,Japan4.International Joint Laboratory for Drug Target of Critical Illnesses,School of Pharmacy,Nanjing Medical University,Nanjing,Jiangsu 211166,China5.Department of Physiology,School of Basic Medical Sciences,Nanjing Medical University,Nanjing,Jiangsu 211166,China6.Gusu School,Nanjing Medical University,Suzhou,Jiangsu 215001,China7.International Joint Laboratory for Drug Target of Critical Illnesses,School of Pharmacy,Nanjing Medical University,Nanjing,Jiangsu 211166,China;Gusu School,Nanjing Medical University,Suzhou,Jiangsu 215001,China;National Vaccine Innovation Platform,Nanjing Medical University,Nanjing,Jiangsu 211166,China
摘要：We have developed a protein array system,named"Phospho-Totum",which reproduces the phosphorylation state of a sample on the array.The protein array contains 1 471 proteins from 273 known signaling pathways.According to the activation degrees of tyrosine kinases in the sample,the corresponding groups of substrate proteins on the array are phosphorylated under the same conditions.In addition to measuring the phosphorylation levels of the 1 471 substrates,we have developed and performed the artificial intelligence-assisted tools to further characterize the phosphorylation state and estimate pathway activation,tyrosine kinase activation,and a list of kinase inhibitors that produce phosphorylation states similar to that of the sample.The Phospho-Totum system,which seamlessly links and interrogates the measurements and analyses,has the potential to not only elucidate pathophysiological mechanisms in diseases by reproducing the phosphorylation state of samples,but also be useful for drug discovery,particularly for screening targeted kinases for potential drug kinase inhibitors.
机标关键词：intelligencescreeningproteintoolschipwithartificialcombined
分类号：R91(药物基础科学)
论文发表日期：2024-05-30
在线出版日期：2026-03-31（本平台首次上网日期，不代表文献的发表时间）
页数：12( 前插1,195-205 )
英文信息