Indirubin alleviates retinal neurodegeneration through the regulation of PI3K/AKT signaling
Huan Li
Huiying Zhang
Lushu Chen
Yaming Shen
Yuan Cao
Xiumiao Li
Jin Yao
Department of Ophthalmology,the Affiliated Eye Hospital of Nanjing Medical University,Nanjing,Jiangsu 210029,China;Department of Ophthalmology,the Fourth School of Clinical Medicine,Nanjing Medical University,Nanjing,Jiangsu 210029,China
摘要：Retinal neurodegenerative disease is a leading cause of blindness among the elderly in developed countries,including glaucoma,diabetic retinopathy,traumatic optic neuropathy and optic neuritis,etc.The current clinical treatment is not very effective.We investigated indirubin,one of the main bioactive components of the traditional Chinese medicine Danggui Longhui Pill,in the present study for its role in retinal neurodegeneration.Indirubin exhibited no detectable tissue toxicity in vivo or cytotoxicity in vitro.Moreover,indirubin improved visual function and ameliorated retinal neurodegeneration in mice after optic nerve crush injury in vivo.Furthermore,indirubin reduced the apoptosis of retinal ganglion cells induced by oxidative stress in vitro.In addition,indirubin significantly suppressed the increased production of intracellular reactive oxygen species and the decreased activity of superoxide dismutase induced by oxidative stress.Mechanically,indirubin played a neuroprotective role by regulating the PI3K/AKT/BAD/BCL-2 signaling.In conclusion,indirubin protected retinal ganglion cells from oxidative damage and alleviated retinal neurodegeneration induced by optic nerve crush injury.The present study provides a potential therapeutic medicine for retinal neurodegenerative diseases.
机标关键词：throughneurodalleviatesdegenerationindirubinregulationretinalsignaling
分类号：R774(视网膜及视神经疾病)
论文发表日期：2024-05-30
在线出版日期：2026-03-31（本平台首次上网日期，不代表文献的发表时间）
页数：14( 中插6,256-268 )
英文信息