Human circBOULE RNAs as potential biomarkers for sperm quality and male infertility
Liping Cheng1
He Jin1
Tianheng Xiao1
Xiaoyu Yang2
Tingting Zhao1
Eugene Yujun Xu3
1.State Key Laboratory of Reproductive Medicine and Offspring Health,Nanjing Medical University,Nanjing,Jiangsu 211166,China2.Center for Clinical Reproduction,the First Affiliated Hospital with Nanjing Medical University&Jiangsu Province Hospital,Nanjing,Jiangsu 210029,China3.State Key Laboratory of Reproductive Medicine and Offspring Health,Nanjing Medical University,Nanjing,Jiangsu 211166,China;Cellular Screening Center,the University of Chicago,Chicago,IL 60637,USA
摘要：Reliable molecular biomarkers to predict fertility remain scarce.The current study investigated the potential of testis-specific circBOULE RNAs as biomarkers for male infertility and sperm quality.Using reverse transcription-PCR and real-time reverse transcription-PCR assays,we identified seven circular RNAs from the human BOULE gene in human sperm.We observed that the expression level of circEx3-6 was significantly reduced in asthenozoospermia,while the expression levels of both circEx2-6 and circEx2-7 were decreased in terato-zoospermia,compared with the controls.Furthermore,we demonstrated that the expression level of circEx2-6 was negatively correlated with the sperm DNA fragmentation index,and the expression level of circEx2-7 was correlated with both fertilization and cleavage rates in those treated with the assisted reproductive technologies.Further functional analyses in a transgenic fly model supported the roles of circBOULE RNAs in sperm development and human male fertility.Collectively,our findings support that sperm circBOULE RNAs may serve as diagnostic biomarkers for assessing sperm motility and DNA quality.Therefore,clinical application and significance of sperm circBOULE RNAs in the assisted reproductive technologies warrant further investigation.
机标关键词：qualityhumanspermrnasbiomarkerscircbouleinfertilitymale
分类号：R321.1(人体形态学)
论文发表日期：2024-09-30
在线出版日期：2026-03-31（本平台首次上网日期，不代表文献的发表时间）
页数：13( 中插5,473-484 )
英文信息