GSDMD protects intestinal epithelial cells against bacterial infections through its N-terminal activity affecting intestinal immune homeostasis
Honghui Li1
Jie Pu2
Dongxue Yang1
Lu Liu1
Yingchao Hu1
Shuo Yang1
Bingwei Wang2
1.Department of Immunology,State Key Laboratory of Reproductive Medicine,Jiangsu Key Lab of Cancer Biomarkers,Prevention and Treatment,Collaborative Innovation Center for Personalized Cancer Medicine,Gusu School,the Affiliated Wuxi People's Hospital of Nanjing Medical University,Wuxi People's Hospital,Wuxi Medical Center,Nanjing Medical University,Nanjing,Jiangsu 211166,China2.Department of Pharmacology,Nanjing University of Chinese Medicine,Nanjing,Jiangsu 210023,China
摘要：The intestinal mucosal barrier serves as a vital guardian of the gut health,maintaining a delicate equilibrium between gut microbiota and host immune homeostasis.Gasdermin D(GSDMD),a key executioner of pyroptosis downstream of the inflammasome,has been found to play intricate roles in modulating colitis by influencing intestinal macrophages and regulating mucus secretion from goblet cells.However,the exact nature of the regulatory function of GSDMD in maintaining intestinal immune homeostasis and defending against pathogens remains to be elucidated.In the current study,by using the Citrobacter rodentium infection model,we found that GSDMD played a key role in the defense against intestinal Citrobacter rodentium infection,with high expression levels in intestinal epithelial and lamina propria myeloid cells.Our results showed that GSDMD acted specifically in intestinal epithelial cells to combat the infection,independently of its effects on antimicrobial peptides or mucin secretion.Instead,the resistance was mediated by the N-terminal fragment of GSDMD,highlighting its importance in intestinal immunity.However,the specific mechanism underlying the N-terminal activity of GSDMD in protecting against intestinal bacterial infections requires future investigation.
机标关键词：activitythroughgsdmdcellsaffectingagainstbacterialepithelial
分类号：R392.1(医学免疫学)
论文发表日期：2024-11-30
在线出版日期：2026-03-31（本平台首次上网日期，不代表文献的发表时间）
页数：13( 中插6,585-596 )
英文信息