The role of SRPK1-mediated phosphorylation of SR proteins in the chromatin configuration transition of mouse germinal vesicle oocytes
Xia Wang1
Shuai Zhou2
Haojie Yin1
Jian Han1
Yue Hu1
Siqi Wang1
Congjing Wang1
Jie Huang1
Junqiang Zhang2
Xiufeng Ling2
Ran Huo1
1.State Key Laboratory of Reproductive Medicine and Offspring Health,Department of Histology and Embryology,Suzhou Affiliated Hospital of Nanjing Medical University,Suzhou Municipal Hospital,Gusu School,Nanjing Medical University,Nanjing,Jiangsu 211166,China2.Department of Reproductive Medicine,Women's Hospital of Nanjing Medical University,Nanjing Women and Children's Healthcare Hospital,Nanjing,Jiangsu 210004,China
摘要：Meiotic resumption in mammalian oocytes involves nuclear and organelle structural changes,notably the chromatin configuration transition from a non-surrounding nucleolus(NSN)to surrounding nucleolus(SN)in germinal vesicle oocytes.In the current study,we found that nuclear speckles(NSs),a subnuclear structure mainly composed of serine-arginine(SR)proteins,changed from a diffuse spotted distribution in mouse NSN oocytes to an aggregated pattern in SN oocytes.We also found that the SR protein-specific kinase 1(SRPK1),an enzyme that phosphorylates SR proteins,co-localized with NSs at the SN stage,and that NSN oocytes failed to transition to SN oocytes after the inhibition of SRPK1 activity.Furthermore,the typical structure of the chromatin ring around the nucleolus in SN oocytes collapsed after treatment with an SRPK1 inhibitor.Mechanistically,phosphorylated SR proteins were found to be related to chromatin as shown by a salt extraction experiment,and in situ DNase I assay showed that the accessibility of chromatin was enhanced in SN oocytes when SRPK1 was inhibited,accompanied by a decreased repressive modification on histone and the abnormal recurrence of a transcriptional signal.In conclusion,our results indicated that SRPK1-regulated phosphorylation of SR proteins was involved in the NSN-SN transition and played an important role in maintaining the condensed nucleus of SN oocytes via interacting with chromatin.
机标关键词：phosphorylationoocytesvesiclemouserolechromatinconfigurationgerminal
分类号：R321.1(人体形态学)
论文发表日期：2025-03-30
在线出版日期：2026-03-31（本平台首次上网日期，不代表文献的发表时间）
页数：12( 中插7,198-208 )
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