Integrated analysis of colorectal cancer metastasis identifies characteristics of tumor cell during metastasis
Haoyu Fu1
Xiaohuan Lu1
Tiantian Ji2
Liping Wang3
Guobin Wang4
Lin Wang3
Zheng Wang1
1.Department of Gastrointestinal Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei,P.R.China;Research Center for Tissue Engineering and Regenerative Medicine,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei,P.R.China;Hubei Key Laboratory of Regenerative Medicine and Multi-disciplinary Translational Research,Wuhan,Hubei,P.R.China;Hubei Provincial Engineering Research Center of Clinical Laboratory and Active Health Smart Equipment,Wuhan,Hubei,P.R.China2.Research Center for Tissue Engineering and Regenerative Medicine,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei,P.R.China;Hubei Key Laboratory of Regenerative Medicine and Multi-disciplinary Translational Research,Wuhan,Hubei,P.R.China;Hubei Provincial Engineering Research Center of Clinical Laboratory and Active Health Smart Equipment,Wuhan,Hubei,P.R.China3.Research Center for Tissue Engineering and Regenerative Medicine,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei,P.R.China;Hubei Key Laboratory of Regenerative Medicine and Multi-disciplinary Translational Research,Wuhan,Hubei,P.R.China;Hubei Provincial Engineering Research Center of Clinical Laboratory and Active Health Smart Equipment,Wuhan,Hubei,P.R.China;Department of Clinical Laboratory,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei,P.R.Chin4.Department of Gastrointestinal Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei,P.R.China
摘要：Background:Metastasis is the main cause of death in colorectal cancer(CRC).Metastasis is a sequential and dynamic process,but the development of tumor cells during this process is unclear.In this study,we aimed to reveal characteristics of tumor cell subset during CRC metastasis.
Methods:Single-cell RNA sequence CRC data of normal epithelium,non-metastatic primary tumor,metastatic primary tumor,and liver metastases from gene expression omnibus(GEO)dataset were analyzed to reveal characteristics of CRC metastasis.Primary tu-mor tissues of three non-metastatic CRC and three metastatic CRC patients from Union Hospital of Tongji Medical College(Wuhan,China)were used to verify the characteristics of CRC metastasis.
Results:We identified a metastasis-related cancer cell subset EP1,which was characterized with a high expression of KRT17,LAMC2,EMP1,and PLAC8.EP1 had an enhanced cell-cell interaction,which interacted with SPPþ macrophages and drove them toward anti-inflammatory and immunosuppressive phenotype.Dynamic changes in genes and TF regulons during the metastasis were also revealed.
Conclusions:This study advanced our understanding of the development of tumor cells during CRC metastasis and further identi-fied metastasis-related subset and potential therapeutic targets for the treatment and prevention of CRC metastasis.
机标关键词：characteristicsintegratedanalysiscancertumorcellcolorectalduring
论文发表日期：2024-10-30
在线出版日期：2026-07-17（本平台首次上网日期，不代表文献的发表时间）
页数：10( 497-506 )
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