DOI: 10.1515/mr-2021-0013
Application of third-generation sequencing in cancer research
Zhiao Chen1
Xianghuo He2
1.Fudan University Shanghai Cancer Center and Institutes of Biomedical Sciences,Fudan University,302 Rm.,7# Bldg,270 Dong An Road,Shanghai 200032,China;Department of Oncology,Shanghai Medical College,Fudan University,Shanghai,China2.Fudan University Shanghai Cancer Center and Institutes of Biomedical Sciences,Fudan University,302 Rm.,7# Bldg,270 Dong An Road,Shanghai 200032,China;Department of Oncology,Shanghai Medical College,Fudan University,Shanghai,China;Key Laboratory of Breast Cancer in Shanghai,Fudan University Shanghai Cancer Center,Fudan University,Shanghai,China
摘要：In the past several years,nanopore sequencing technology from Oxford Nanopore Technologies(ONT)and single-molecule real-time(SMRT)sequencing tech-nology from Pacific BioSciences(PacBio)have become available to researchers and are currently being tested for cancer research.These methods offer many advantages over most widely used high-throughput short-read sequencing approaches and allow the comprehensive analysis of transcriptomes by identifying full-length splice isoforms and several other posttranscriptional events.In addition,these platforms enable structural variation characterization at a previously unparalleled resolution and direct detection of epigenetic marks in native DNA and RNA.Here,we present a comprehensive summary of important applications of these technologies in cancer research,including the identification of com-plex structure variants,alternatively spliced isoforms,fusion transcript events,and exogenous RNA.Further-more,we discuss the impact of the newly developed nanopore direct RNA sequencing(RNA-Seq)approach in advancing epitranscriptome research in cancer.Although the unique challenges still present for these new single-molecule long-read methods,they will unravel many as-pects of cancer genome complexity in unprecedented ways and present an encouraging outlook for continued application in an increasing number of different cancer research settings.
机标关键词：applicationgenerationresearchcancersequencingthird
论文发表日期：2021-04-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：22( 150-171 )
英文信息