DOI: 10.1515/mr-2023-0050
Engineering pluripotent stem cells with synthetic biology for regenerative medicine
Yihuan Mao1
Siqi Wang1
Jiazhen Yu2
Wei Li3
1.State Key Laboratory of Stem Cell and Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing,China;Key Laboratory of Organ Regeneration and Reconstruction,Chinese Academy of Sciences,Beijing,China;Beijing Institute for Stem Cell and Regenerative Medicine,Beijing,China2.State Key Laboratory of Stem Cell and Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing,China;Key Laboratory of Organ Regeneration and Reconstruction,Chinese Academy of Sciences,Beijing,China;University of Chinese Academy of Sciences,Beijing,China3.State Key Laboratory of Stem Cell and Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China;Key Laboratory of Organ Regeneration and Reconstruction,Chinese Academy of Sciences,Beijing 100101,China;Beijing Institute for Stem Cell and Regenerative Medicine,Beijing 100101,China;University of Chinese Academy of Sciences,Beijing 100049,China
摘要：Pluripotent stem cells(PSCs),characterized by self-renewal and capacity of differentiating into three germ layers,are the programmable building blocks of life.PSC-derived cells and multicellular systems,particularly organoids,exhibit great potential for regenerative medicine.However,this field is still in its infancy,partly due to limited strategies to robustly and precisely control stem cell behaviors,which are tightly regulated by inner gene regu-latory networks in response to stimuli from the extracellular environment.Synthetic receptors and genetic circuits are powerful tools to customize the cellular sense-and-response process,suggesting their underlying roles in precise control of cell fate decision and function reconstruction.Herein,we review the progress and challenges needed to be overcome in the fields of PSC-based cell therapy and multicellular system generation,respectively.Furthermore,we summa-rize several well-established synthetic biology tools and their applications in PSC engineering.Finally,we highlight the challenges and perspectives of harnessing synthetic biology to PSC engineering for regenerative medicine.
机标关键词：pluripotentengineeringmedicinebiologystemcellswithregenerative
论文发表日期：2024-04-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：20( 90-109 )
英文信息