Rosmarinic acid ameliorates hypoxia/ischemia induced cognitive deficits and promotes remyelination
Man Li1
Miao-Miao Cui1
Nwobodo Alexander Kenechukwu1
Yi-Wei Gu2
Yu-Lin Chen1
Si-Jing Zhong3
Yu-Ting Gao4
Xue-Yan Cao2
Li Wang2
Fu-Min Liu1
Xiang-Ru Wen5
1.Department of Genetics, Xuzhou Medical University, Xuzhou, Jiangsu Province, China2.Department of Urology, the First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China3.Xuzhou Medical University Clinical Medical College, Xuzhou, Jiangsu Province, China4.Department of Clinical Laboratory, Xuzhou Medical University, Xuzhou, Jiangsu Province, China5.Research Center for Neurobiology and Department of Neurobiology, Xuzhou Medical University, Xuzhou, Jiangsu Province, China
摘要：Rosmarinic acid,a common ester extracted from Rosemary,Perilla frutescens,and Salvia miltiorrhiza Bunge,has been shown to have protective effects against various diseases.This is an investigation into whether rosmarinic acid can also affect the changes of white matter fibers and cognitive deficits caused by hypoxic injury.The right common carotid artery of 3-day-old rats was ligated for 2 hours.The rats were then prewarmed in a plastic container with holes in the lid,which was placed in 37℃ water bath for 30 minutes.Afterwards,the rats were exposed to an atmosphere with 8％ O2 and 92％ N2 for 30 minutes to establish the perinatal hypoxia/ischemia injury models.The rat models were intraperitoneally injected with rosmarinic acid 20 mg/kg for 5 consecutive days.At 22 days after birth,rosmarinic acid was found to improve motor,anxiety,learning and spatial memory impairments induced by hypoxia/ischemia injury.Furthermore,rosmarinic acid promoted the proliferation of oligodendrocyte progenitor cells in the subventricular zone.After hypoxia/ischemia injury,rosmarinic acid reversed to some extent the downregulation of myelin basic protein and the loss of myelin sheath in the corpus callosum of white matter structure.Rosmarinic acid partially slowed down the expression of oligodendrocyte marker Olig2 and myelin basic protein and the increase of oligodendrocyte apoptosis marker inhibitors of DNA binding 2.These data indicate that rosmarinic acid ameliorated the cognitive dysfunction after perinatal hypoxia/ischemia injury by improving remyelination in corpus callosum.This study was approved by the Animal Experimental Ethics Committee of Xuzhou Medical University,China (approval No.20161636721) on September 16,2017.
机标关键词：
分类号：R453(治疗学)R741(神经病学)R842.1(潜水医学)
论文发表日期：2020-05-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：9( 894-902 )
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