Study on Preparation of the Low-Molecular-Weight Chitosan Using Cellulase
LI He-sheng
SUN Yu-xi
HUANG Xiao-chun
WANG Hong-fei
QIU Di-hong
摘要：The degradation of chitosan ( DD of 72.05%) with aid of cellulase was carried out under the conditions of 45℃, pH 5.0 and a ratio of 1:15( chitosan/enzyme). The results showed cellulase could degrade chitosan efficiently. Viscosity of chitosan was decreased very quickly and reducing sugar released was increased with time during degradation. By using the membrane, the separation of the hydrolysis mixture was studied. Rejection of protein can be reached to be 99.74%.65.9% of low-molecular-weight chitosans was less than 2 kDa. Solubility of low-molecular-weight chitosan was found to be better than chitosan and transmittance could reach to be more than 95 % in entire range of pH 1 ～ 13.
机标关键词：reducing sugarrange of
分类号：R3(基础医学)
论文发表日期：2006-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 113-120 )
英文信息