The Effect of Bioceramic Composite Extracellular Matrixes Used to Repair Bone Deficiency on Relevant Blood Biochemical Indices

摘要：At the base of experimental animal model construction of bone defect in New Zealand rabbit, the promoting repair effect of bioactive ceramics on bone defect as well as its machanism was studied through testing body mineral elements, enzymes related to bone morphogenetic proteins and some biochemical indexes. Refering to some documents, materials of TCP, CHA and HA were combined and TCP/BMP/ TCP-β1 and CHA/BMP/ TCP-β1, HA/BMP/ TCP-β1 composite materials were made. All kinds of them were implanted into the radial defect site of rabbit, respectively. The chosen blood indexes (Ca, P, ALP, GGT, AST, ALT, TPA, BUN and Cr) were tested by colorimetry, speed rate and bromocresol green testing methods. No abnormal effects were found in any animal after operation. Serum concentrations of Ca, P and ALP were increased with the length of time in all groups of the three kinds of composite material, mixed material and pure materials. The increases in composite material groups were more significant ( P ＜0.05). Comparison of the three kinds of material showed TCP ＞ CHA ＞ HA. There was a tendency of increased TPA and decreased BUN with the length of time. There was no significant difference between the composite material groups and pure material group (P ＞0.05). The three kinds of bioactive ceramics composed of extracellular matrix could increase the serum concentrations of Ca and P and activity of ALP after being implanted into defect bone and showed some repairing capacity. This provided a new area of machanism study of bone defect repair by biomaterials.