Characterization of Side Cell Populations Obtained from Human Amnion Mesenchymal Cells
LI Ning
PIAO Zhengfu
Mamoru Kobayashi
Koji Sasaki
DING Shu-qin
Aiko Kikuchi
Isao Kamo
Norio Sakuragawa
摘要：Human amnion mesenchymal cells (AMCs) contain multipotent cells. To enrich such multipotent stem cells, we applied to AMCs the new method for the isolation of side population (SP) cells used for the enrichment of multipotent stem cells from many tissues. We succeeded in obtaining SP cells from AMCs (AMC-SP cells). AMC-SP cells were found in 0.2% of AMCs, irrespective of the length of pregnant period, ranging from 37 to 40 weeks. Cell cycle analyses uggested that AMC-SP cells belonged to a cell population that proliferated very slowly and/or was in a quiescent state in the amniotic membrane. Upon culturing, they proliferated with 40 to 80 cell doublings. However, they did not form colonies in a soft agarose culture, whereas HepG2 cells, representative human hepatoma cells formed many large colonies. These results suggest that AMC-SP cells that have considerable value for the use of regenerative medicine can be managed safely in vitro.
机标关键词：CellsMesenchymalHumanstem cellshuman hepatoma cellsmesenchymalnew methodCell cycle
分类号：R318.1(医用一般科学)
论文发表日期：2009-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 1-8 )
英文信息