Irrelevance of BKCa Channel Expression to VSMCs Phenotype under Shear Stress
JIA Xiao-ling
YANG Jing-yun
YANG Liu
LI Ping
SONG Wei
FAN Yu-bo
摘要：Large conductance Ca2+-activated K+ (BKCa) channel exhibits a phenotype-dependent expression on vascular smooth muscle cells (VSMCs), which prefers to contractile phenotype. Meanwhile, shear stress definitely influences VSMCs proliferation and contraction. Thereby, a hypothesis was raised, would shear stress change the BKCa expression and correlate with VSMC phenotype? In order to investigate it, VSMCs were exposed to shear stress in a parallel-plate flow chamber with 12 dynes/cm2 for 12 h. Subsequently, the effect of shear stress on VSMC proliferation, BKCa channel expression and contractile phenotype marker, α-smooth muscle cell actin (α-SMA) and smooth muscle myosin heavy chain (SMMHC), was determined by immunofluorescence microscopy, flow cytometery as well as reverse transcription-polymerase chain reaction (RT-PCR), respectively. Data show that shear stress enhanced the expression of BKCa channel while inhibiting VSMC proliferation. Paralleled to those phenomena, the expression of both α-SMA and SM-MHC were decreased significantly. These results demonstrated that upregulation of BKCa channel was irrelevant to the maintenance VSMC of contractile phenotype under shear stress. This finding provides a new insight into understanding the correlation of BKCa channel and VSMC phenotype.
机标关键词：shear stressvascular smooth muscle cellsmyosin heavy chainflow chamber
分类号：R318.01(医用一般科学)
论文发表日期：2016-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：5( 53-57 )
英文信息