Increased Transendothelial Cells Permeability Induced by Oxidized Lipoprotein(a) Relates to Reactive Oxygen Species Generation and Desmogleins Expression
WANG Zuo
YANG Jian
ZHANG Xiao-lei
MA Xiao-feng
WANG Ren
ZENG Jun-fa
WEI Deng-heng
ZHANG Kai
LI Shuang
LIN Xiao-long
ZHAO Yue
JIANG Zhi-sheng
摘要：Objective: This paper aims at measurement enhanced effect of oxidized lipoprotien(a) [oxLp(a)] on permeability of monolayer endothelial cells and relationship with reactive oxygen species(ROS) generation and desmogleins(DSGs) expression. Methods and Results: Transendothelial permeability was assayed by transwell and reactive oxygen species (ROS) was determined by DCFH-DA staining. RT-PCR was carried out to determine DSGl and DSC2 expression in mRNA, respectively. Transendothelial permeability was enhanced by oxLP (a) dose and time dependently. The most marked effect appeared at a concentration of 100 mg/L, Transendothelial permeability reached the maximum value after 2 h of FITC-dextran addition, and then gradually decreased after 4 h. oxLp (a) induces the generation of cellular reactive oxygen species (ROS), and this effect could be inhibited by superoxide dismutase (SOD). Incubation of HUVECs with oxLp (a) resulted in a dose and time-dependent down-regulation of DSGl and DSC2 expression at transcriptional level. Conclusion: Permeability of monolayer endothelial cells was enhanced by oxLp(a) which is related to up-regulating ROS formation and down-regulating desmogleins expression.
机标关键词：reactive oxygen speciesendothelial cellssuperoxide dismutasemaximum value
分类号：R363.2(病理学)
资助基金：Visiting Scholar Foundation of Key Laboratory of Biorheological Science and Technology (Chongqing University) of Ministry of Education(2010)
论文发表日期：2016-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：7( 127-133 )
英文信息