ISOLATION AND INDUCTION OF DIFFERENTIATION OF SEINE ADIPOSE-DERIVED MESENCHYMAL STEM CELLS
MA Yueying
YUAN Shuolong
ZHANG yue
XU liangwei
GUO Weiwei
ZHAO Lidong
ZHAI suoqiang
YANG Shiming
摘要：Objectives To establish a method for high yield mesenchymal stem cells collection, as well as a culture method for iden-tifying mesenchymal stem cells from the swine adipose-derived mesenchymal stem cell (ADMSC). Methods Swine AD-MSCs were isolated from fat tissue with collagenase, followed by induction of differentiation to osteogenic, adipogenic and chondrogrnic cells. The survival curve of the ADMSC at the 37oC and 38oC were measured using WST-1Cell Proliferation As-say Reagent. Result ADMSCs isolated with collagenase from swine neck fat tissue generated a stable uniform appearance af-ter the second generation. The passage period was five days. ADMSC could differentiate into osteogenic, adipogenic or chon-drogrnic cells under different culture conditions. The highest growth rate was achieved at 38oC in this study. <br> Conclusion Swine ADMSCs have the potential to differentiate into osteogenic, adipogenic or chondrogrnic cells, and they may be appropriate for transplantation for both research and clinical purpose.
机标关键词：mesenchymal stem cellsfat tissueculture conditionssecond generationsurvival curvegrowth ratehigh yield
资助基金：grants from the National Basic Research Program of China (973Program)(2012CB967900)grants from the National Basic Research Program of China (2012CB967901)
论文发表日期：2014-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：5( 101-105 )
英文信息