Role of the periotic mesenchyme in the development of sensory cells in early mouse cochlea
Jingjiang Huang
Na Zuo
Cheng Wu
Peipei Chen
Jun Ma
Chuanxi Wang
Wenyan Li
Shaofeng Liu
摘要：Objective: To investigate the role of the periotic mesenchyme (POM) in the development of sensory cells of developing auditory epithelium.Methods: Developing auditory epithelium with or without periotic mesenchyme was isolated from mice at embryonic days 11.5 (E11.5), E12.5 and E13.5, respectively, and cultured in vitro to an equivalent of E18.5's epithelium in vivo. Then, the explants were co-stained with antibodies targeting myosin VIIA, Sox2 and BrdU. Results: More hair cells in E11.5+7 DIV, E12.5+6 DIV and E13.5+5 DIV auditory epithelia were found upon culture with POM (225.90 ± 62.44, 476.94 ± 100.81, and 1386.60 ± 202.38, respectively) compared with the non-POM group (68.17 ± 23.74, 205.00 ± 44.23, and 1266.80 ± 38.84, respectively). Moreover, regardless of developmental stage, the mesenchymal tissue increased the amount of cochlear sensory cells as well as the ratio of differentiated hair cells to total sensory cells. Conclusions: The periotic mesenchyme promotes the development of cochlear sensory cells, and its ef-fect depends on the developmental stage of the auditory epithelium.
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资助基金：This work was supported by the Chinese National Natural Science Foundation of China(grant number81371089)
论文发表日期：2020-12-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：6( 138-143 )
英文信息