Exploring the bioactive substances and mechanisms of Chuanxiong Rhizoma treating acute lung injury based on combined network pharmacological,phytochemical,and pharmacological investigations
Lintao Xu
Xinping Cheng
Qingwen Hu
Guangcheng Peng
Xinyu Qi
Yu Zhao
Xiaoning Wang
Zhenpeng Xu
Tao Shen
摘要：Background:Acute lung injury(ALI)is a high-fatality respiratory disease,and the development of new therapeutic agents is ongoing.Chuanxiong Rhizoma has been prescribed for the therapy of ALI in traditional Chinese medicine.
Objective:The purpose of this article is to explore the effective ingredients,targets,and mechanisms in the treatment of Chuanxiong Rhizoma on ALI.
Methods:The active ingredients of Chuanxiong Rhizoma were obtained in the TCMSP,and their potential targets and ALI targets were predicted in TCMSP,Swiss target prediction,and Genecards database.A PPI network was constructed in the string online platform,and KEGG and GO enrichment analysis were conducted in the metascape online platform.Phytochemical investigation of Chuanxiong Rhizoma was performed with chemical separation methods and structural elucidation techniques,and their pharmacological effects were evaluated using LPS-induced mouse ALI model and cell-based assays.
Results:Network pharmacology analyses indicated that the treatment of Chuanxiong Rhizoma on ALI was associated with its anti-inflammatory effect.The key genes mainly include TNF,STAT3,EGFR,AKT1,BCL2,CASP3,and SRC,and the active compounds are its signature component,phthalides.Phytochemical investigation led to the elucidation of 23 phthalides,which demonstrated an anti-inflammatory effect in vitro and in vivo.Of which,chuanxiongdiolide A,levistilide A,butylphthalide,and senkyunolide A were indeed effective in mitigating ALI in an LPS-induced mouse model.
Conclusions:The combined network pharmacological,phytochemical,and pharmacological investigations revealed that the therapeutical effect of Chuanxiong Rhizoma against ALI was associated with inhibition of the inflammatory response by phthalides.
机标关键词：chuanxiongsubstancesnetworkacutebasedbioactivecombinedexploring
论文发表日期：2024-12-30
页数：13( 182-194 )
英文信息