摘要： This paper aims to the research of the impact of fluid shear stress on the adhesion between vascular endothelial cells and leukocyte induced by tumor necrosis factor-α(TNF-α) by microfliudic chip technology. Microfluidic chip was fabricated by soft lithograph;Endothelial microfluidic chip was constructed by optimizing types of the extracellular matrix proteins modified in the microchannel and cell incubation time;human umbilical vein endothelial cells EA.Hy926 lined in the microchannel were exposed to fluid shear stress of 1.68 dynes/cm2 and 8.4 dynes/cm2 respectively. Meanwhile, adhesion between EA.Hy926 cells and leukocyte was induced by TNF-αunder a flow condition. EA. Hy926 cell cultured in the static condition was used as control group. The numbers of fluorescently-labeled leukocyte in microchannel were counted to quantize the adhesion level between EA. Hy926 cells and leukocyte; cell immunofluorescence technique was used to detect the intercellular adhesion molecule (ICAM-1) expression. The constructed endothelial microfluidic chip can afford to the fluid shear stress and respond to exogenous stimulus of TNF-α;compared with the adhesion numbers of leukocyte in control group, adhesion between EA. Hy926 cells exposed to low fluid shear stress and leukocyte was reduced under the stimulus of TNF-α at a concentration of 10 ng/ml(P<0.05);leukocyte adhesion with EA. Hy926 cells exposed to high fluid shear stress was reduced significantly than EA. Hy926 cells in control group and EA.1Hy926 cells exposed to low fluid shear stress ( P<0.01); the regulation mechanism of fluid shear stress to the adhesion between EA. Hy926 cells and leukocyte induced by TNF-αwas through the way of ICAM-1. The endothelial microfluidic chip fabricated in this paper could be used to study the functions of endothelial cell in vitro and provide a new technical platform for exploring the pathophysiology of the related cardiovascular system diseases under a flow environment.

摘要：This paper aims to meet the requirements of reducing the scanning time of magnetic resonance imaging (MRI), accelerating MRI and reconstructing a high quality image from less acquisition data as much as possible. MRI method based on compressed sensing (CS) with multiple regularizations (two regularizations including total variation (TV) norm and L1 norm or three regularizations consisting of total variation, L1 norm and wavelet tree structure) is proposed in this paper, which is implemented by applying split augmented lagrangian shrinkage algorithm (SALSA). To solve magnetic resonance image reconstruction problems with linear combinations of total variation and L1 norm, we utilized composite split denoising (CSD) to split the original complex problem into TV norm and L1 norm regularization subproblems which were simple and easy to be solved respectively in this paper. The reconstructed image was obtained from the weighted average of solutions from two subproblems in an iterative framework. Because each of the splitted subproblems can be regarded as MRI model based on CS with single regularization, and for solving the kind of model, split augmented lagrange algorithm has advantage over existing fast algorithm such as fast iterative shrinkage thresholding(FIST) and two step iterative shrinkage thresholding (TwIST) in convergence speed. Therefore, we proposed to adopt SALSA to solve the subproblems. Moreover, in order to solve magnetic resonance image reconstruction problems with linear combinations of total variation, L1 norm and wavelet tree structure, we can split the original problem into three subproblems in the same manner, which can be processed by existing iteration scheme. A great deal of experimental results show that the proposed methods can effectively reconstruct the original image. Compared with existing algorithms such as TVCMRI, RecPF, CSA, FCSA and WaTMRI, the proposed methods have greatly improved the quality of the reconstructed images and have better visual effect.

摘要：To investigate the influence of the furnace structure and inlet gas velocity on gas-solid two-phase flow states in the deposition furnace,FLUENT software was used to simulate dense gas-solid two-phase flow in a fluidized bed.The results show that the dispersed fluidized bed formed in the deposition furnace with a cone angle of 60° has the structure of"ring-nucleus"and longer gas retention time compared with the flat-bottom structure deposition furnace,which is beneficial to the preparation of the pyrolytic carbon coating materials with a uniform structure and excellent quality.Inlet gas velocity should be moderate,the slow inlet gas velocity leads to the chaos of the particle velocity distribution,which cannot form the ring-core flow structure.High inlet gas velocity leads to the back mixing of particles in the deposition furnace.In addition,as the inlet gas velocity increases,both the gas phase and the average particle velocity in the central area of the deposition furnace gradually increase,and the effect of the gas phase velocity is more obvious.

摘要：Hyaluronan (HA), the consistent glycosaminoglycans in extracellular matrix, is a kind of biomaterials with wonderful biocompatibility. To develop drug release system (DDS) with HA as drug carrier is a new hotspot in the field of pharmaceutics. In this paper, we applied technique of ultrosound and reversed phase (Water/Oil) emulsification to develop dexamethasone (DEX)-HA-STMP cross-linking microspheres (DEX-HA MS) with STMP as cross-linker. DEX-HA MS has a wonderful shape and property of dispersion. There is a negative correlation between diameter of DEX-HA MS and the content of cross-linker, or the content of emulsifier, and a positive correlation between the diameter and CHA. When CHA≤1% ,DEX/HA≤1/10(g/g),there is a positive correlation between the factors mentioned below and drug loading (DL%)/loading efficiency (LE%) ,the content of STMP, the content of emulsifier, CHA and the content of DEX. DEX-HA-MS can realize function of slow release. In vitro drug release experiment shows that cumulative release (CR%) of DEX-HA MS fits in with pervasion-corrosion equation, and there is a negative correlation between the content of STMP, CHA and CR% , a positive correlation between emulsifier and CR%. When DEX/HA ≤1/5 (g/g) there is a negative correlation between the content of DEX and CR%.

摘要：Interface debonding between prostheses and abutments was the most frequent failure mode of resin-bonded fixed partials dentures (RBFPDs) in clinic. The purpose of this study was to investigate the effect of accessory retention forms on the bond strength of RBFPDs. Three types of 3D finite element models were constructed. The model of posterior metal plate RBFPD with spoon-shaped occlusal rest seats served as the control. The remaining two types of models based on the control added the retention form design of the pin hole and axial groove respectively. The axial or buccolingual load of 150 N was applied on the prosthesis, first premolar and first molar respectively. The maximum principal stresses of the adhesive layer in different models were calculated. Under the load of the same magnitude, the stress due to the buccolingual load was significantly higher than that due to the axial load in the adhesive layer. The proximal shoulder, occlusal rest seats wall and the proximal margin adjacent to the shoulder were the high risk region where the adhesive layer damaged easily. Compared with the control model, the pin and groove models could slightly decrease the stress in the adhesive layer after the axial loading, while the stress in the adhesive layer drastically decreased after horizontal loading and reduced by 22% and 31%respectively. These results indicate that the horizontal occlusal force has a more serious harm to the debonding of RBFPDs. In addition, the accessory retention forms (e.g. pin and axial groove retention forms) can decrease the stress level in the adhesive layer, which are conducive to increase the load-bearing capacity of RBFPDs.

摘要：This paper presents the analysis and management system of digital ultrasonic image. The system can manage medical ultrasonic image by collecting, saving and transferring, and realize that section offices of ultrasonic image in hospital network manage. The system use network technology in transferring image between ultrasonic equipments to share patient data in ultrasonic equipments. And doctors can input patient diagnostic report,saved by text file and case history, digitally managed. The system can be realized by Visual C++ which make windows applied. The system can be brought forward because PACS prevail with various hospitals,but PACS is expensive. In view of this status, we put forward to the analysis and management system of digital ultrasonic image,which is similar to PACS.

摘要： Calcium phosphate ceramics are widely used as biomaterials to substitute or/and repair hard tissues because of their excellent biocompatibility and bioactivity. Among them, hydroxyapatite (HA) is the most promising material. However, their mechanical brittleness restrains them from being used as load-bearing implants.

摘要： Cell affinity is an important factor to be concerned when biodegradable polymeric materials are utilized as cell scaffold in tissue engineering . Many studies haveproved that hydrophilicity , surface energy, charge and roughness of the materialsurface greatly influence the cell attachment and cell growth on the material.

摘要：Objective: To make statistics on the distribution of ApoE gene polymorphism in the Longyan area, provide data for the statistics of regional cerebrovascular diseases, explore the significance of ApoE gene polymorphism distribution for clinical guidance, diagnosis and treatment, and provide more suggestions for clinical diagnosis and treatment of cerebrovascular diseases. Methods: 800 patients with cerebrovascular disease in the Longyan area from July 2018 to April 2021 were selected as the research object,and 800 normal healthy patients were selected as the control group. The data were further analyzed according to the collected patient information, and the patients were distinguished according to age and other basic information, the type of cerebrovascular disease, ApoE gene polymorphism and blood lipid test results. The distribution of ApoE gene polymorphism in the cerebrovascular disease population in the Longyan area was given to determine the correlation between the incidence of cerebrovascular disease and the distribution of ApoE gene polymorphism. Results:In the study group, E2/E3 genotyping was the most common 400 (50.00％), and E4/E4 genotyping 6 (0.75％) was the least common;In the control group, E2/E4 genotyping was the most common 480(60.00％), and E2/E3 genotyping accounted for the second 290 (36.25％). In the study group, E3 allele was the most common 383 (47.88％),E4 allele 121 (15.12％);in the control group, E2 allele was the most common 391 (48.88％), and E3 allele 142 (17.75％). Conclusion: According to the statistics of ApoE gene polymorphism distribution in the Longyan area and the analysis of the correlation of other cerebrovascular diseases,it is found that E2/E3 genotype may be related to cerebrovascular diseases, and E3 allele may be a susceptibility factor. Through the above analysis, the occurrence of diseases can be predicted and prevented.

摘要：A frequency following response (FFR) of speech auditory brainstem response (speech-ABR) elicited by the speech syllable/da/contains three distinct waves named as D, E and F, corresponding to the structure of the stimulus sound. The detection and characterization of FFRs are critical in the study and application of speech-ABRs. Conventional methods detect the latencies of the waves in time domain by measuring the maximal amplitudes of the waveform in the preset windows, which suffers the problem of low quality of FFR waves. In this paper, we defined an instantaneous energy (IE) spectrum based on empirical mode decomposition (EMD) method (EMD-IE method) to detect FFR and measured the latencies of the waves. The results reveal that the FFRs are mostly evident on the second layer of the IE spectra, which would benefit the detection and measurement of the FFRs in clinic.

摘要：The recognition of electroencephalogram (EEG) signals is the key of brain computer interface (BCI).Aimed at the problem that the recognition rate of EEG by using support vector machine (SVM) is low in BCI,based on the assumption that a well-defined physiological signal which also has a smooth form"hides" inside the noisy EEG signal,a Quasi-Newton-SVM recognition method based on Quasi-Newton method and SVM algorithm was presented.Firstly,the EEG signals were preprocessed by Quasi-Newton method and got the signals which were fit for SVM.Secondly,the preprocessed signals were classified by SVM method.The present simulation results indicated the Quasi-Newton-SVM approach improved the recognition rate compared with using SVM method; we also discussed the relationship between the artificial smooth signals and the classification errors.

摘要：Objective: To explore the diagnostic value of tb-RNA in pleural effusion in tuberculous pleurisy. Methods: 60 patients with tuberculous pleurisy treated from March 2018 to September 2019 were selected as the research object, and 60 patients with non-tuberculous pleurisy treated at the same time were selected as the control group to analyze the diagnostic consistency of tuberculous pleurisy. Results: Through the consistency analysis of the gold standard and tb-RNA diagnosis, the diagnosis consistency of the two methods was strong. By comparing the tb-RNA level of the two groups, the tb-RNA level of the observation group was significantly higher than that of the control group (P<0.05); Through the analysis of diagnostic efficacy of tb-RNA, the diagnostic sensitivity and specificity of tb-RNA were 58.33％and 97.50％, respectively. Conclusion: tb-RNA detection of pleural effusion has a high positive diagnostic value and short detection cycle, which is of positive significance for the diagnosis of tuberculous pleural effusion.

摘要：This paper presents a novel method of spot addressing and segmentation about the foreground segmentation of microarray image.In this paper,a spot addressing method based on particle swarm optimization (PS O),algorithm is proposed to have a further search for the center coordinate and radius of the spot whose region is determined by the projection method.Then,a foreground segmentation method is put forward to make the spot foreground segmentation based on the center coordinate and radius of the spot.The spot addressing and segmentation experiments on synthetic and real microarray images show that the proposed method is effective and feasible for the foreground segmentation of microarray image.

摘要： One of the challenges in the field of tissue engineering is the development of biomaterial/cell interactions. For this purpose, the use of biological materials in tissue engineering requires the application of different chemical or physical procedures for surface modification in order to improve the cell affinity of biomaterials. We have studied the effects of one chemical treatment and one physical treatment: 1-(3-dimethylaminopropyl )-3-ethylcarbodiimide (WSC) and entrapment.

摘要： Glioma has the highest incidence in the brain tumors. Though treated with surgical resection, external beam radiation therapy, and systemic chemotherapy, patients with glioma have poor prognosis because of glioma recurrence.

摘要：In biology ferment engineering, accurate statistics of the quantity of bacte-ria is one of the most important subjects. In this paper, the quantity of bacteria which was observed traditionally manuauy can be detected automatically. Image acquisition and pro-cessing system is designed to accomplish image preprocessing, image segmentation and statistics of the quantity of bacteria. Segmentation of bacteria images is successfully real-ized by means of a region-based level set method and then the quantity of bacteria is com-puted precisely, which plays an important role in optimizing the growth conditions of bac-teria.

摘要：Developing a shear thinning non-Newtonian fluid to substitute blood is desirable in studies of biomedicine engineering since blood is a shear thinning nonNewtonian fluid that exhibits both viscous and elastic properties, and generally not available in large volume. The rheological properties of aqueous Xanthan gum with different concentrations and temperatures were studied in present paper. The results show that aqueous Xanthan gum is a non-Newtonian fluid which displays remarkable shear thinning behaviour and is a suitable blood analog fluid. There is a power law distribution relationship between shear stress and shear rate, and the higher the solution concentration is, the more strongly it displays shear thinning behaviours. Viscosity values of aqueous Xanthan gum increase with the solution temperature decrease or with the solution concentration increase in linearity. Moreover at the temperature of 37℃, aqueous Xanthan gum with concentration of 0.4‰ and 0.6‰ matches human blood best in rheological properties. According to the resuits, the viscosity expression varied with temperature and concentrition is obtained, and the stability of Xanthan gum solution is discussed.